Cholera toxin is an oligomeric protein of MW 84kD and consists of a single A subunit surrounded by five B subunits. It is a potent activator of adenylate cyclase and is the pathogenic agent responsible for the symptoms of cholera. The B subunit (choleragenoid) is responsible for the binding of the holotoxin to G M1 ganglioside receptors on mammalian cell surfaces and facilitates entrance of the A subunit into the cell. The A subunit bears the ADP-ribosyl-transferase activity, which deregulates the G sprotein causing activation of adenylate cyclase. Due to the ubiquitous occurrence of the GM1 ganglioside receptor on eukaryotic cell membranes, cholera toxin activates adenylate cyclase in a wide variety of systems. Cholera toxin has become a powerful research tool not only in microbiology, but in the fields of physiology, cell biology and biochemistry, as well. Because of the effect on adenylate cyclase, cholera toxin and its purified A subunit are frequently used for the study of signal transduction mechanisms. In addition, cholera toxin acts as an adjuvant through the stimulation of B-lymphocytes. The cholera toxin B subunit alone is used for track tracing in neurological research, taking advantage of GM1 ganglioside binding and retrograde transport.

Suitable for use in FLISA and Immunohistochemistry. Other applications have not been tested.

Immunohistochemistry: Frozen sections Optimal dilutions to be determined by the researcher.

Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight™550 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Note: Applications are based on unconjugated antibody.

Vibrio cholera

Supplied as a liquid in PBS, pH 7.2. Labeled with MaxLight™550.

Purified by Protein G affinity chromatography

Recognizes the labile entertoxin normally associated with Vibrio cholerae and reacts with an epitope on the beta chain. No crossreactivity with various E. coli serotypes.