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C5815-10M-APC Mouse Anti-c-Jun (jun oncogene, Activator protein 1, AP1, AP-1, c-Jun, p39, Proto-oncogene c-jun, Transcription Factor AP-1, V-jun Avian Sarcoma Virus 17 Oncogene Homolog) (APC)

Specifications
Clone Type
Monoclonal
Host
Mouse
Source
Human
Swiss Prot
P45983
Conjugate
APC
Isotype
IgG1,k
Clone Number
6E4.4
Grade
Affinity Purified
Applications
IC WB
Crossreactivity
Hu Mo Rt
Accession #
NP_002219.1
Shipping Temp
Blue Ice
Storage Temp
4°C Do Not Freeze
Notes
BSA Free

c-Jun is a component of the transcription factor AP-1 that binds and activates transcription at TRE/AP-1 elements and appears to be a major downstream target of the SAPK/JNK signaling pathway. The transcriptional activity of c-Jun is regulated by phosphorylation at Ser63 and Ser73. Extracellular signals including growth factors, transforming oncoproteins and UV irradiation stimulate phosphorylation of c-Jun at Ser63/73 and activate c-Jun dependent transcription. Mutation of Ser63/73 renders c-Jun nonresponsive to mitogenic and stress induced signaling pathways. The MAP kinase homologue, SAPK/JNK, binds to the N-terminal region of c-Jun and phosphorylates c-Jun at Ser63/73. In addition, the activity of SAPK/JNK is stimulated by the same signals that activate c-Jun.

Applications
Suitable for use in Western Blot and Immunocytochemistry. Other applications not tested.
Recommended Dilution
Western Blot: 0.5-2ug/ml Optimal dilutions to be determined by the researcher.
Positive Control
NIH/3T3 cell lysate
Storage and Stability
Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: APC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Note: Applications are based on unconjugated antibody.
Immunogen
GST-tagged recombinant protein corresponding to c-Jun.
Form
Supplied as a liquid in PBS, pH 7.2. Labeled with Allophycocyanin (APC).
Purity
Purified by Protein G affinity chromatography.
Specificity
Recognizes human c-Jun, regardless of phosphorylation state. Species Crossreactivity: mouse and rat.
Conjugates
Pricing
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