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C5837-02C Rabbit Anti-CLC1 (Clcn1, Chloride Channel)

Specifications
References
Clone Type
Polyclonal
Host
Rabbit
Source
Rat
Isotype
IgG
Grade
Affinity Purified
Applications
E
Crossreactivity
Rt
Shipping Temp
Blue Ice
Storage Temp
4°C (-20°C Glycerol)

Chloride is a critical component of all living cells. Voltage-gated chloride channels regulate cellular traffic of chloride ion. The chloride channels (CIC or CLC) performs several functions including the regulation of cell volume, membrane potential stabilization, signal transduction, and transepithelial transport. Mutations in CIC genes have been linked with several human diseases including myotonias (Thomsen's disease), cystic fibrosis, Bartters syndrome type III, Dent's disease, and X-linked recessive nephrolithiasis. In mammals, CLC proteins form a superfamily of at least 9 different genes (CLC1-7 also known as CLCN1-7 and CLK1-2 or CLCKa and CLCKb). Additional forms of these proteins are obtained by alternative splicing. All CLC proteins (~700-1000 aa) are predicted to contain 10 (possibly 12) transmembrane domains.Except CLC-1 and CLC-K1/K2 that are specific for kidney, most other CLC are widely distributed in various tissues. Rat CLC-1 is 994 aa membrane protein (human CLC-1 988 aa) (1). It is predominantly expressed in skeletal muscles. Defects in CLC1 (CLCN1) are the cause of autosomal recessive generalized myotonia (Becker's disease) (RGM) and autosomal dominant myotonia congenita (Thomsen's disease; MC) which are characterized by skeletal muscle stiffness (delayed relaxation) that is a result of muscle membrane hyperexcitability.

Applications
Suitable for use in ELISA. Western Blot, though not tested, may potentially be used as an application. Other applications not tested.
Recommended Dilution
Western Blot: 1-10ug/ml using ECL. ELISA: 0.5-1ug/ml. Use 1ug/ml of peptide to coat ELISA plates. Optimal dilutions to be determined by the researcher.
Control Peptide
C5837-02E: CLC1, Rat (Clcn1, Chloride Channel).
Storage and Stability
May be stored at 4°C for short-term only. For long-term storage, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Immunogen
18aa Peptide sequence near the C-terminus of rat CLC-1. (KLH coupled). Species sequence homology: mouse, canine and human 100%. No significant sequence homology is detected with other CLCs or other proteins.
Form
Supplied as a liquid in PBS, pH 7.4 and 0.1% BSA, before the addition of 40% glycerol.
Purity
Purified by immunoaffinity chromatography.
Specificity
Recognizes rat CLC1.
References
1. Steinmeyer K et al (1991) Nature 354, 301; 2. Koch MC et al (1992) Science 257, 797; 3. George AL et al (1993) Nature Genet. 3, 305; 4. Steinmeyer K et al (1994) EMBO J 13, 737; 5. Lorenz C et al (1994) Human Mol. Genet. 3, 941; 6. Heine R et al (1994) Hum. Mol. Genet. 3, 1123; 7. George Al et al (1994) Hum. Mol. Genet. 3, 2071; 8. Meyer-Kleine C et al (1995) Am. J. Hum. Genet. 57, 1325; 9. Lehman Horn F et al (1995) Hum. Mol. Genet 4, 1397; 10. Mailander V et al (1996) ) Am. J. Hum. Genet. 58, 317
USBio References
No references available
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