Suitable for use in Immunofluorescence, FLISA and Western Blot. Not suitable for use in Immunohistochemistry. Other applications not tested.

Optimal dilutions to be determined by the researcher.

Cartilage

Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Note: Applications are based on unconjugated antibody.

Purified preparation of lathyritic Type II Collagen from embryonic chicken sternum. Cellular Localization: Catilage matrix.

Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein isothiocyanate (FITC).

Purified by Protein A affinity chromatography.

Recognizes chicken Collagen Type II. Show no crossreactivity with Types I, III, IV, V, VI, IX, X or XI. In Western Blot, reacts with the TCA fragment of lathyritic Type II Collagen after digestion with mammalian collagenase. It does not react with pepsin-digested Type II Collagen. The epitope recognized is GGFDEK, slightly preceding the lysine residue involved in crosslinking. Two terminal glycine residues constitute a key structural element of the epitope. The epitope is lost after digestion of lathyritic Type II Collagen with recombinant Stromelysin. |Species Crossreactivity: human and mouse.