This gene encodes an alpha chain for one of the low abundance fibrillar collagens. Fibrillar collagen molecules are trimers that can be composed of one or more types of alpha chains. Type V collagen is found in tissues containing type I collagen and appears to regulate the assembly of heterotypic fibers composed of both type I and type V collagen. This gene product is closely related to type XI collagen and it is possible that the collagen chains of types V and XI constitute a single collagen type with tissue-specific chain combinations. Mutations in this gene are associated with Ehlers-Danlos syndrome, types I and II.
Applications
Suitable for use in RIA, Immunofluorescence, Immunohistochemistry and ELISA. Other applications not tested.
Recommended Dilution
Immunohistochemistry: 1:40 for immunofluorescent staining of frozen human skin, liver and placenta tissues. Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Extracted and purified from human placenta
Form
Supplied as a liquid in 0.1M citrate, 0.1M potassium phosphate, pH 7.2-7.4, 10ug/ml of antibiotics and antimytotics
Specificity
Recognizes human Collagen Type V. Shows less than 0.1% reactivity with human Collagen Types I, III, and IV. C7510-55K also shows 0.1% reactivity with human Fibronectin and mouse Laminin by radioimmunoassay. Crossreactivity: Human collagen type I, II, & III: < 0.5%, Human collagen type IV: 20%, Human fibronectin, albumin, immunoglobulins: < 0.5%.