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C7905-09D-PE Mouse Anti-C-Peptide (PE)

Specifications
Clone Type
Monoclonal
Host
Mouse
Source
Rat
Conjugate
PE
Isotype
IgG1,k
Clone Number
5E542
Grade
Affinity Purified
Applications
FLISA RIA
Crossreactivity
Hu
Shipping Temp
Blue Ice
Storage Temp
4°C Do Not Freeze
Notes
Preservative Free
BSA Free

C-Peptide is part of the molecule of Proinsulin, that consists of three parts: C-Peptide and two long strands of amino acids (called the alpha and beta chains) that later become linked together to form the insulin molecule. From every molecule of proinsulin, one molecule of insulin plus one molecule of C-Peptide are produced. C-Peptide is released into the blood stream in equal amounts to insulin. A test of C-Peptide levels will show how much insulin the body is making. Insulin decreases blood glucose concentration. It increases cell permeability to monosaccharides, amino acids and fatty acids. It accelerates glycolysis, the pentose phosphate cycle, and glycogen synthesis in liver. Although C-Peptide function is not fully understood, it binds to cell membranes which leads to increased intracellular Ca2++, K+ -ATPase and endothelial nitric oxide synthase activities. C-Peptide also functions in repair of the muscular layer of arteries.

Applications
Suitable for use in FLISA and RIA. Other applications have not been tested.
Recommended Dilution
Optimal dilutions to be determined by the researcher.
Hybridoma
X63-Ag8-653 myeloma cells with spleen cells of Balb/c mice immunized with recombinant C-peptide construction.
Affinity Constant
(Kd) > 1x10e8 for all clones.
Storage and Stability
Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Note: Applications are based on unconjugated antibody.
Immunogen
Recombinant C-peptide
Form
Supplied as a liquid in PBS, pH 7.2. No preservative added. No stabilizing proteins added. Labeled with R-Phycoerythrin (PE).
Purity
Purified by Protein A affinity chromatography. >90% Purity is tested by electrophoresis.
Specificity
Recognizes human free C-peptide and C-peptide region in proinsulin molecules. Antigenic determinants identified by Mab are in the three-dimensional structure generated by N- and C-terminal regions of C-peptide separated by beta-turn at position 47-50 of proinsulin. Does not crossreact with human, bovine, porcine and mouse/rat insulin.
Conjugates
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