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C8455-01K Rabbit Anti-CCNE1, CT (G1/S-specific Cyclin-E1, CCNE)

Specifications
References
Clone Type
Polyclonal
Host
Rabbit
Source
Human
Swiss Prot
P24864
Isotype
IgG
Grade
Affinity Purified
Applications
FC IHC WB
Crossreactivity
Hu
Accession #
NP_001229
Shipping Temp
Blue Ice
Storage Temp
-20°C

Cyclin E1 belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance through the cell cycle. Cyclins function as regulators of CDK kinases. Different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. Cyclin E1 forms a complex with and functions as a regulatory subunit of CDK2, whose activity is required for cell cycle G1/S transition. Cyclin E1 accumulates at the G1-S phase boundary and is degraded as cells progress through S phase. Overexpression of Cyclin E1 has been observed in many tumors, which results in chromosome instability, and thus may contribute to tumorigenesis. This protein was found to associate with, and be involved in, the phosphorylation of NPAT protein (nuclear protein mapped to the ATM locus), which participates in cell-cycle regulated histone gene expression and plays a critical role in promoting cell-cycle progression in the absence of pRB.

Applications
Suitable for use in Immunohistochemistry, Flow Cytrometry and Western Blot. Other applications have not been tested.
Recommended Dilution
Immunohistochemistry (formalin-fixed, parafin-embedded): 1:50-1:100 Flow Cytrometry: 1:10-1:50 Western Blot: 1:50-1:100 Optimal dilutions to be determined by researcher.
Positive Control
A2058 cell line lysates, human testis tissue and NCI-H292 cells.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Synthetic peptide corresponding to the C-terminal region of human Cyclin E1 conjugated to KLH (P24864).
Form
Supplied as a liquid in PBS, 0.09% sodium azide.
Purity
Purified by immunoaffinity chromatography.
Specificity
Recognizes human Cyclin E1 at ~47kD.
References
1.Ausserlechner, M.J., et al., Leukemia 19(6):1051-1057 (2005). 2.Wingate, H., et al., J. Biol. Chem. 280(15):15148-15157 (2005). 3.Honda, R., et al., EMBO J. 24(3):452-463 (2005). 4.Brzezinski, J., et al., Clin. Cancer Res. 11(3):1037-1043 (2005). 5.Hayami, R., et al., Cancer Res. 65(1):6-10 (2005).
USBio References
No references available
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