APAP1
DEDAF (death effector domain associated factor), is a 32kD protein that associates with DED-containing proteins but does not itself contain a DED. DEDAF interacts with the death effector domains of FADD, DEDD and the proforms of caspase-8 and caspase-10. It augments the formation of complexes between CD95, FADD and caspase-8 at the cell membrane and enhances apoptosis. The protein was also independently identified as RING1 and YY1 binding protein (RYBP), which was implicated in the regulation of transcription.
Applications
Suitable for use in Western Blot. Other applications not tested.
Recommended Dilutions
Western Blot: A band at ~32kD was detected in human A549 cell lysates. Optimal dilutions to be determined by the researcher.
Storage and Stability
Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Note: Applications are based on unconjugated antibody.
Immunogen
Synthetic peptide corresponding to aa215-228 of human DEDAF.
Form
Supplied as a liquid in PBS, pH 7.2. Labeled with horseradish peroxidase (HRP).
Purity
Purified by affinity chromatography from whole serum.
Specificity
Recognizes human death effector domain associated factor (DEDAF). Species Crossreactivity: mouse and rat
TDS
NCBI
UniProt
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