DEDAF (death effector domain associated factor), is a 32kD protein that associates with DED-containing proteins but does not itself contain a DED. DEDAF interacts with the death effector domains of FADD, DEDD and the proforms of caspase-8 and caspase-10. It augments the formation of complexes between CD95, FADD and caspase-8 at the cell membrane and enhances apoptosis. The protein was also independently identified as RING1 and YY1 binding protein (RYBP), which was implicated in the regulation of transcription.

Suitable for use in Western Blot. Other applications not tested.

Western Blot:A band at ~32kD was detected in human A549 cell lysates. Optimal dilutions to be determined by the researcher.

Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight™490 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Note: Applications are based on unconjugated antibody.

Synthetic peptide corresponding to aa215-228 of human DEDAF.

Supplied as a liquid in PBS, pH 7.2. Labeled with MaxLight™490.

Purified by affinity chromatography from whole serum.

Recognizes human death effector domain associated factor (DEDAF). Species Crossreactivity: mouse and rat