DAGLA

Clone Type

Polyclonal

Host

Goat

Source

Human

Isotype

IgG

Grade

Affinity Purified

Applications

E WB

Crossreactivity

Hu Mo Rt

Accession #

NP_006124.1

Gene ID

747, 269060, 309207

Shipping Temp

Blue Ice

Storage Temp

-20°C

Catalyzes the hydrolysis of diacylglycerol (DAG) to 2-arachidonoyl-glycerol (2-AG), the most abundant endocannabinoid in tissues. Required for axonal growth during development and for retrograde synaptic signaling at mature synapses.

Applications

Suitable for use in ELISA and Western Blot. Other applications not tested.

Recommended Dilution

ELISA: 1:128,000 Western Blot: 0.3-1ug/ml Optimal dilutions to be determined by the researcher.

Storage and Stability

May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Immunogen

Synthetic peptide corresponding to C-PAKQDELVISAR, from human DAGLA, at C-terminal (NP_006124.1).

Form

Supplied as a liquid in Tris saline, 0.02% sodium azide, pH 7.3, 0.5% BSA.

Purity

Purified by immunoaffinity chromatography.

Specificity

Recognizes human DAGLA. Species sequence homology: Mouse and rat.

References

1. Bisogno T, Howell F, Williams G, Minassi A, Cascio MG, Ligresti A, Matias I, Schiano-Moriello A, Paul P, Williams EJ, Gangadharan U, Hobbs C, Di Marzo V, Doherty P. Cloning of the first sn1-DAG lipases points to the spatial and temporal regulation of endocannabinoid signaling in the brain. J Cell Biol. 2003 Nov 10;163(3):463-8.

USBio References

No references available

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D3224-60A Goat Anti-DAGLA (Sn1-specific Diacylglycerol Lipase alpha, DGL-alpha, Neural Stem Cell-derived Dendrite Regulator, C11orf11, KIAA0659, NSDDR)

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