3ß Hydroxy-5-androsten-17-one, 3ß Hydroxyandrost-5-en-17-one, Androstenol, Androstenolone, Dehydroisoandrosterone, Hydroxyandrost-5-en-17-one, Trans Dehydroandrosterone
DHEA is produced primarily by the adrenal glands, and together with its ester DHEA-sulphate (DHEAS), has been implicated in a wide range of physiological roles including aging, immunology, memory and obesity. In particular, DHEA and DHEAS differentially regulate growth-factor induced proliferation of chromaffin cells, and are thought to affect neurogenesis, neuronal survival, and neuronal stem cell proliferation. Blood measurements of DHEAS/DHEA are useful to detect excess adrenal activity due to adrenal cancer or hyperplasia, including certain forms of congenital adrenal hyperplasia.
Applications
Suitable for use in ELISA. Other applications not tested.
Recommended Dilution
ELISA: 1:1,000-1:10,000 Optimal dilutions to be determined by the researcher.
Recommended Secondary Antibodies
I1904-06C: IgG, H&L (HRP) (X-Adsorbed) Pab Gt xMo I1904-06H IgG, H&L (HRP) (X-Adsorbed) Pab Gt xMo I1904-14C: IgG, Fc (HRP) Pab Gt xMo
Storage and Stability
Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Note: Applications are based on unconjugated antibody.
Immunogen
DHEA-3-HS-BSA conjugate
Form
Supplied as a liquid in PBS, pH 7.2. Labeled with horseradish peroxidase (HRP).
Purity
Purified by Protein A affinity chromatography.
Specificity
Recognizes Dehydroepiandrosterone.
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