DHEA is the most abundant hormone in the human body and a precursor of all the sex hormones. It is synthesised principally in the adrenal glands and gonads from cholesterol, through conversion to pregnenolone and 17a-Hydroxypregnenolone, by the action of P450 enzymes. DHEA is mainly found in serum as the sulphated form (DHEAS), a reversible reaction catalysed by sulfotransferase in the adrenal glands, liver and small intestine.

Suitable for use in FLISA. Other applications not tested.

Optimal dilutions to be determined by the researcher.

Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight™490 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Note: Applications are based on unconjugated antibody.

DHEA-3-HS-BSA

Supplied as a liquid in PBS, pH 7.2. Labeled with MaxLight™490.

Purified by Protein A affinity chromatography.

Recognizes Dehydroepiandrosterone Sulfate. This antibody was raised against DHEA but demonstrates greater reactivity with DHEAS. Crossreactivity: DHEAS: 100%, DHEA: 13%, Progesterone: <0.01%, Testosterone: 0.02%. Broad species reactivity is expected.