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D3520-01R Rabbit Anti-Dicer (Endoribonuclease Dicer, Helicase with RNase Motif, Helicase MOI, DICER1, HERNA, KIAA0928) (Not for Export EU)

Specifications
References
Clone Type
Polyclonal
Host
Rabbit
Source
Human
Isotype
IgG
Grade
Serum
Applications
E FC
Crossreactivity
Hu
Accession #
NP_803187
Shipping Temp
Blue Ice
Storage Temp
-20°C

Dicer is an RNase III-like enzyme which cleaves double-stranded RNA into 21-23 nt RNAs. These RNAs are referred to as siRNA (short interfering RNA). It is believed to be essential in siRNA-mediated gene suppression.

Applications
Suitable for use in ELISA and Flow Cytometry. Other applications not tested.
Recommended Dilution
ELISA: 1:200 Flow Cytometry (Intracellular): 1:100-1:1000 Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Synthetic peptide corresponding to aa1423-1440 of human Dicer.
Form
Supplied as a liquid, 0.025% sodium azide, 50% glycerol.
Purity
Serum.
Specificity
Recognizes human Dicer.
References
1. Matsuda,S., Ichigotani,Y., Okuda,T., Irimura,T., Nakatsugawa,S. and Hamaguchi,M. Molecular cloning and characterization of a novel human gene (HERNA) which encodes a putative RNA-helicase Biochim. Biophys. Acta 1490 (1-2), 163-169 (2000) 2. Nagase,T., Ishikawa,K., Suyama,M., Kikuno,R., Hirosawa,M., Miyajima,N., Tanaka,A., Kotani,H., Nomura,N. and Ohara,O. Prediction of the coding sequences of unidentified human genes. XIII. The complete sequences of 100 new cDNA clones from brain which code for large proteins in vitro DNA Res. 6 (1), 63-70 (1999).
USBio References
No references available
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