Dopa decarboxylase (DDC), also known as AADC, is a homodimeric vitamin B 6-dependent enzyme that catalyzes the decarboxylation of hydroxylated aromatic amino acids (Accession # P20711). DDC is expressed in the adrenal medulla and catecholaminergic neurons. It catalyzes the final step in the synthesis of the neurotransmitters dopamine and serotonin. Defects in DDC are associated with the development of schizophrenia, Parkinson's disease, and hypertension. Human DDC shares 89% aa sequence identity with mouse and rat DDC.
Applications
Suitable for use in Western Blot and ELISA. Other applications not tested.
Recommended Dilution
Western Blot: 2ug/ml. Detects human DDC in lysates of Jurkat human acute T cell leukemia cell line KO Validated: Dopa Decarboxylase/DDC is specifically detected in Jurkat human acute T cell leukemia parental cell line but is not detectable in Dopa Decarboxylase/DDC knockout Jurkat cell line. Optimal dilutions to be determined by the researcher.
Storage and Stability
Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Immunogen
Sf21-derived recombinant protein corresponding to Met1-Glu480 from human DDC.. Species Sequence Homology: mouse and rat, 89%
Form
Supplied as a lyophilized powder from PBS, 5% trehalose. Reconstitute with 200ul sterile PBS.
Purity
Purified by Protein G affinity chromatography from hybridoma culture supernatant.
Specificity
Recognizes human DDC.
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