Suitable for use in Flow Cytometry, Immunohistochemistry, Immunoprecipitation, RIA and Western Blot. Other applications not tested.

Immunohistochemistry: Frozen, Paraffin and Resin sections Optimal dilutions to be determined by the researcher.

Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Note: Applications are based on unconjugated antibody.

Thioglycollate stimulated peritoneal macrophages from C57/BL mice.

Supplied as a liquid in PBS, pH 7.2. Labeled with Fluorescein isothiocyanate (FITC).

Purified by Protein G affinity chromatography from tissue culture supernatant.

Recognizes mouse F4/80.