Enolase 1 gene encodes one of three enolase isoenzymes found in mammals; it encodes alpha-enolase, a homodimeric soluble enzyme, and also encodes a shorter monomeric structural lens protein, tau-crystallin. The two proteins are made from the same message. The full length protein, the isoenzyme, is found in the cytoplasm. The shorter protein is produced from an alternative translation start, is localized to the nucleus, and has been found to bind to an element in the c-myc promoter.

Suitable for use in Immunofluorescence and Western Blot. Other applications not tested.

Immunofluorescence: 10ug/ml using HeLa cell lysates Western Blot: 1:500-1:1000 Optimal dilutions to be determined by the researcher.

Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight™650 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Note: Applications are based on unconjugated antibody.

Full-length recombinant protein corresponding to aa1-435 of human ENO1, with a GST Tag. MW of GST Tag alone is 26kD, (P06733).

Supplied as a liquid in PBS, pH 7.2. No preservative added Labeled with MaxLight™650.

Purified

Recognizes human ENO1 at ~47kD.