USBio Logo

E3375-53A Rabbit Anti-Epidermal Growth Factor Receptor, phosphorylated (Tyr1068) (erbB, EGFR)

Specifications
References
Clone Type
Polyclonal
Host
Rabbit
Source
Human
Swiss Prot
P00533
Isotype
IgG
Grade
Affinity Purified
Applications
E IHC WB
Crossreactivity
Hu Mo Rt
Gene ID
1956
Shipping Temp
Blue Ice
Storage Temp
-20°C
Notes
Preservative Free

The epidermal growth factor (EGF) receptor is a 170kD transmembrane tyrosine kinase that belongs to the HER/ErbB protein family. Ligand binding results in receptor dimerization, autophosphorylation, activation of downstream signaling and lysosomal degradation. Phosphorylation of EGF receptor (EGFR) at Tyr845 in the kinase domain is implicated in stabilizing the activation loop, maintaining the active state enzyme and providing a binding surface for substrate proteins. c-Src is involved in phosphorylation of EGFR at Tyr845. The SH2 domain of PLCgamma binds at phospho-Tyr992, resulting in activation of PLCgamma-mediated downstream signaling. Phosphorylation of EGFR at Tyr1045 creates a major docking site for c-Cbl, an adaptor protein that leads to receptor ubiquitination and degradation following EGFR activation. The GRB2 adaptor protein binds activated EGFR at phospho-Tyr1068. A pair of phosphorylated residues (Tyr1148 and Tyr1173) provides a docking site for the SHC scaffold protein, with both sites involved in MAP kinase signaling activation. Phosphorylation of EGFR at specific serine and threonine residues attenuates EGFR kinase activity. EGFR carboxy-terminal residues Ser1046 and Ser1047 are phosphorylated by CaM kinase II; mutation to either of these serines results in upregulated EGFR tyrosine autophosphorylation.

Applications
Suitable for use in ELISA, Western Blot and Immunohistochemistry. Other applications have not been tested.
Recommended Dilutions
Western Blot: 1:1000 Immunohistochemistry (Paraffin): 1:350 Use EDTA as the unmasking buffer. Dilute E3375-53A in TBST, 5% NGS. Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Synthetic phosphopeptide corresponding to residues surrounding Tyr1068 of human EGF receptor.
Form
Supplied as a liquid in 10mM HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol. No preservative added.
Purity
Purified by Protein A and peptide affinity chromatography.
Specificity
Recognizes endogenous levels of human EGF receptor at ~175kD only when phosphorylated at Tyr1068. May recognize other activated EGF receptor family members (ErbB2) and crossreacts slightly with activated PDGF receptor. Species Crossreactivity: mouse and rat
References
1. Hackel, P.O. et al. (1999) Curr Opin Cell Biol 11, 184-9. 2. Zwick, E. et al. (1999) Trends Pharmacol Sci 20, 408-12. 3. Cooper, J.A. and Howell, B. (1993) Cell 73, 1051-4. 4. Hubbard, S.R. et al. (1994) Nature 372, 746-54. 5. Biscardi, J.S. et al. (1999) J Biol Chem 274, 8335-43. 6. Emlet, D.R. et al. (1997) J Biol Chem 272, 4079-86. 7. Levkowitz, G. et al. (1999) Mol Cell 4, 1029-40. 8. Ettenberg, S.A. et al. (1999) Oncogene 18, 1855-66. 9. Rojas, M. et al. (1996) J Biol Chem 271, 27456-61. 10. Feinmesser, R.L. et al. (1999) J Biol Chem 274, 16168-73.
USBio References
No references available
Conjugates
Pricing
Order
Proceed to Checkout
Cart Summary
ProductSizeListYour PriceQtyExt Price
Subtotal:Subtotal:
Subtotal:Subtotal:
Total Coupon Savings:Total Coupon Savings:()
Your cart is currently empty.
- Coupon Code
Recently Viewed
Contact Us

Visit our technical library or contact our support staff to answer your questions.

Telephone:
1-800-520-3011

Library | Contact

Distributors

For customers outside of the United States, please use one of our many distributors.

View Distributors

Payment Methods

We accept the following payment methods as well as pay-by-invoice.

MasterCard Visa PayPal
© 2023-2024 United States Biological - All Rights Reserved