Applications
Suitable for use in Immunofluorescence and ELISA. Other applications not tested.
Recommended Dilutions
Optimal dilutions to be determined by the researcher.
Matched Pairs
Capture: E3500-37J Detection: E3500-37L Capture: E3500-37L Detection: E3500-37H
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Partially purified E. coli O157 LPS
Form
Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide
Purity
Purified by Protein A affinity chromatography from ascites
Specificity
Recognizes the “O” antigen of E. coli serotype O157. Crossreactivities: Salmonella O30 (Hilversum) and Salmonella O30 (urbana). Non-reactive with E. coli serotypes O111, O125, O20 and O55