Suitable for use in Immunofluorescence and FLISA. Other applications not tested.

Optimal dilutions to be determined by the researcher.

Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Note: Applications are based on unconjugated antibody.

Partially purified E. coli O157 LPS

Supplied as a liquid in PBS, pH 7.2. No preservative added. No stabilizing proteins added. Labeled with Fluorescein isothiocyanate (FITC).

Purified by Protein A affinity chromatography from ascites

Recognize the “O” antigen of E. coli serotype O157. Crossreactivities: Salmonella O30 (Hilversum) and Salmonella O30 (urbana). Non-reactive with E. coli serotypes O111, O125, O20 and O55