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E3500-37L-ML550 Mouse Anti-Escherichia coli, O157 (E. coli) (MaxLight 550)

Specifications
Brand
MaxLight™
Clone Type
Monoclonal
Host
Mouse
Source
E. coli
Conjugate
MaxLight™550
Isotype
IgG2b
Clone Number
10J22
Grade
Affinity Purified
Applications
FLISA IF
Crossreactivity
Ec
Shipping Temp
Blue Ice
Storage Temp
4°C Do Not Freeze
Notes
Preservative Free

MaxLight™550 is a new Yellow-Green photostable dye conjugate comparable to Alexa Fluor™546, 555, DyLight™549 , Cy3™, TRITC and offers better labeling efficiency, brighter imaging and increased immunodetection. Absorbance (550nm); Emission (575nm); Extinction Coefficient 150,000.

Applications
Suitable for use in Immunofluorescence and FLISA. Other applications not tested.
Recommended Dilutions
Optimal dilutions to be determined by the researcher.
Storage and Stability
Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight™550 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Note: Applications are based on unconjugated antibody.
Immunogen
Partially purified E. coli O157 LPS
Form
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight™550.
Purity
Purified by Protein A affinity chromatography from ascites
Specificity
Recognize the “O” antigen of E. coli serotype O157. Crossreactivities: Salmonella O30 (Hilversum) and Salmonella O30 (urbana). Non-reactive with E. coli serotypes O111, O125, O20 and O55
Conjugates
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