Estradiol is a steroid hormone secreted by developing follicles of the ovary. The determination of estradiol concentrations in the body fluids is of great value for endocrinological investigations in women. This antibody may also prove useful in identification of ovarian tumors.
Applications
Suitable for use in ELISA. Other applications not tested.
Recommended Dilutions
Optimal dilutions to be determined by the researcher.
Storage and Stability
Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Note: Applications are based on unconjugated antibody.
Immunogen
Estradiol-6-CMO-BSA
Form
Supplied as a liquid in PBS, pH 7.2. No preservative added. No stabilizing proteins added. Labeled with Horseradish peroxidase (HRP).
Purity
Purified by Protein A affinity chromatography from ascites
Specificity
Recognizes human Estradiol. Crossreactivity: 17-B-Estradiol, 100%; Estriol, 2.3%; Estrone, 0.17%; Testosterone, 0.01%; Androstanedione, Cortisone, Cortisol, Progesterone, Corticosterone, <0.005%.