Baculovirus Expression Vector pAc-k-CH3

Grade

Purified

Shipping Temp

Blue Ice

Storage Temp

-20°C

Baculovirus cassette vector pAc-k-CH3 for the expression of human, humanized or chimeric IgG(kappa) in insect cells and secretion of assembled antibodies into the supernatant. The vector derived from pAcUW51 is suitable for cloning of heavy and light chain Fv antibody gene fragments.

Vector Size

7363bp

Resistance

Vector contains an ampicillin resistance marker gene

Applications

Cloning of heavy and light chain variable domain genes of scFv antibody fragments selected from hybridomas, single B-lymphocytes or phage display libraries.

Cloning sites

Xhol and Nhel for heavy chain VH genes. Sacl and HindIII for light chain VL(k) genes.

Storage and Stability

May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Purity

Purified using a Plasmid Purification Kit.

Concentration

~0.5mg/ml

Form

Supplied as a liquid in TE buffer.

Important Note

This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

Form

Supplied as a liquid in TE buffer.

Purity

Purified using a Plasmid Purification Kit.

References

1. Liang, M., et al., (2001) J. Immunol. Meth. 247:119-130. 2. Liang, M. & Dübel, S., Expresssion of antibody fragments using the Baculovirus system. In: Antibody Engineering, ed: Kontermann, R. and Dübel, S. Springer Verlag, Heidelberg (2001). 3. Barbas, C.F. 3rd, et al., (1991) PNAS USA 88:7978-7982. 4. Nesbit, M., et al., (1992) J. Immunol. Meth. 151:201-208 . 5. Poul, M.A., et al., (1995) Immunotechnology 1:189-196. 6. Poul, M.A., et al., (1995) Eur. J. Immunol. 25:2005-2009. 7. Liang, M., et al., (1997) Virology 235:252-260. 8. Liang, M. & Dübel, S., Expression of antibody fragments using the Baculovirus system. In: Antibody Engineering, ed: Kontermann, R. & Dübel, S., Springer Verlag, Heidelberg (2001). 9. Liang, M., et al., (2001) J. Immunol. Meth. 247:119-130.

USBio References

No references available

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E9073-50 Anti-Baculovirus Expression Vector pAc-k-CH3

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