FADD was originally isolated as a protein that bound to the cytoplasmic domain of Fas in the yeast two-hybrid system. Sequence analysis revealed a region homologous to the death domain of Fas and TNFR-1. Subsequent biochemical studies have shown that FADD associates with Fas through interaction of the death domains. When over expressed in several cell lines, FADD induces apoptosis, which can be blocked by CrmA, an inhibitor of Caspase 1 (ICE). This evidence suggests that FADD plays a role in Fas-mediated apoptosis.

Suitable for use in Western Blot and Immunoprecipitation. Other applications have not been tested.

Western Blot Analysis: 1-2ug/ml detects FADD in RIPA lysates from Jurkat cells. Jurkat cell lysate was resolved by electro-phoresis, transferred to nitrocellulose and probed with F0019-52 (1ug/ml). Proteins were visualized using a goat anti-rabbit secondary antibody conjugated to HRP and a chemiluminescence detection system. Immunoprecipitation: 4ug immunoprecipitates FADD from 500ug of Jurkat cell lysate. Optimal dilutions to be determined by the researcher.

Positive Antigen Control: J9000 Jurkat lysate. Use 20ug per lane for minigels.

May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20°C or colder. Aliquots are stable for at least 24 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Note: Applications are based on unconjugated antibody.

His-tagged full-length fusion protein corresponding to human FADD.

Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight™650.

Purified by Protein A affinity chromatography.

Recognizes human FADD (28kD).