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F0025-06B Mouse Anti-FceRI, alpha (Fc epsilon RI alpha, FCE1A, FCER1A, High Affinity Immunoglobulin epsilon Receptor Subunit alpha, IgE Receptor) (PE)

Specifications
References
Clone Type
Monoclonal
Host
Mouse
Source
Human
Swiss Prot
P12319
Conjugate
PE
Isotype
IgG2b
Clone Number
10C204 (9E1)
Grade
Affinity Purified
Applications
FC
Crossreactivity
Hu
Shipping Temp
Blue Ice
Storage Temp
4°C Do Not Freeze

The human high affinity Fc receptor for IgE (Fc epsilon R1), plays a central role in the IgE-mediated allergic response. Fc epsilon R1 consists of four subunits, a high affinity IgE binding alpha subunit, a beta chain and two di-sulphide linked gamma subunits.

Applications
Suitable for use in Flow Cytometry. Other applications not tested.
Recommended Dilution
Flow Cytometry: Neat; 10ul labels 10e6 cells in 100ul Optimal dilutions to be determined by the researcher.
Recommended Negative Control
I1904-84H3: IgG2b Negative Control, Mouse (PE)
Hybridoma
Sp2/0-Ag14 myeloma cells with spleen cells from Balb/c mice.
Storage and Stability
Store product at 4°C before and after reconstitution. DO NOT FREEZE! Lyophilized and reconstituted products are stable for 12 months at 4°C after receipt. Reconstitute lyophilized powder with sterile ddH2O prior to use and store remainder as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product centrifuge the original vial prior to removing the cap.
Form
Supplied as a lyophilized powder from PBS, 1% BSA, 5% sucrose, 0.09% sodium azide. Labeled with R-Phycoerythrin (PE). Reconstitute with 1ml sterile ddH2O.
Purity
Purified by Protein G affinity chromatography from tissue culture supernatant.
Specificity
Recognizes human FceRI, alpha. Specifically recognizes the extracellular D1 domain of FceRI, alpha. Primarily expressed on mast cells and basophils but expression has also been reported on monocytes, Langerhans cells and dendritic cells from patients with atopic diseases.
References
1. Vangelista, L. et al. (2002) A minimal receptor-Ig chimera of human Fc epsilon R1 alpha-chain efficiently binds secretory and membrane IgE. Protein Engineering. 15: 51-57, 2. Vangelista, L. et al. (2002) Efficient folding of the Fc epsilonR1 alpha-chain membrane-proximal domain D2 depends on the presence of the N-terminal domain D1. J. Mol. Biol. 322: 815-25
USBio References
No references available
Conjugates
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