DYKDDDDK-Tag (ECS, Enterokinase Cleavage Site, FLAG, D Tags)
Applications
Suitable for use in ELISA and Western Blot. Other applications not tested.
Recommended Dilutions
ELISA: 1:9000-1:450,000 Western Blot: 1:2000-1:10,000 Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
KLH conjugated Enterokinase cleavage site peptide DYKDDDDK. Residues of cysteine and glycine were used to facilitate coupling at the C-terminal end.
Form
Supplied as a liquid in 0.02M potassium phosphate, 0.15M sodium chloride, pH 7.2, 0.09% sodium azide.
Purity
Purified by immunoaffinity chromatography.
Specificity
Recognizes the hydrophilic epitope tagging octapeptide sequence DYKDDDDK (Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys), when fused to either the amino- or carboxyl- terminus of target proteins. In Western Blot of bacterial extracts does not cross-react with any endogenous proteins.
References
1. Hopp, T.P. et al. (1988) A short polypeptide marker sequence useful for recombinant protein identification and purification. Bio/Technology 6: 1204-1210. 2. Slootstra, J.W. et al. (1997) Identification of new tag sequences with differential and selective recognition properties for anti-FLAG monoclonal antibodies M1, M2 and M5. Mol. Divers. 2: 156-164.
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