Frizzled-6 is a member of the Frizzled/Smoothened family of G-protein coupled receptors that mediate the Wnt beta-Catenin signaling pathway.
Applications
Suitable for use in Western Blot and Direct ELISA. Other applications not tested.
Recommended Dilution
Western Blot: 1ug/ml with recombinant mouse Frizzled‑6 Optimal dilutions to be determined by the researcher.
Storage and Stability
Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Immunogen
Recombinant protein corresponding to mouse Frizzled-6, His19-Val153 (predicted), expressed in NS0 cells.
Form
Supplied as a lyophilized powder from PBS, pH 7.2, 5% trehalose. Reconstitute with 1ml of sterile PBS.
Purity
Purified by Protein G affinity chromatography from hybridoma culture supernatant.
Specificity
Recongizes mouse Frizzled-6. Does not crossreact with recombinant human Frizzled-5 or recombinant mouse Frizzled-1, -2, -3, -4, -5, -7, -8 or -9.