Forkhead box O (FOXO) transcription factors play an important role in modulating metabolic functions. Given the relatively high expression of FOXO1 in insulin-responsive tissues, this transcription factor is highly poised to regulate energy metabolism. When nutrient and insulin levels are low, FOXO1 promotes expression of gluconeogenic enzymes. Conversely, in the fed state, insulin levels rise and stimulate uptake of glucose primarily into skeletal muscle and other organs, including adipose tissue. Under certain pathophysiologic conditions, including insulin resistance, negative signaling to FOXO1 is compromised. (Gross, D.N., 2009).

Suitable for use in Western Blot, Immunohistochemistry and Immunocytochemistry. Other applications not tested.

Immunohistochemistry (Paraffin): Antigen retrieval with citrate, pH 6.0 is recommended. Optimal dilutions to be determined by the researcher.

HEK293 cell lysate

Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight™550 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Note: Applications are based on unconjugated antibody.

GST-tagged recombinant protein corresponding to full length human FOXO1. Cellular Localization: Cytosol, Nucleus (Shuttles between cytosol and nucleus).

Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight™550.

Purified by Protein G affinity chromatography.

Recognizes human FOXO1 at ~78-82kD. Species Crossreactivity: mouse and rat