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G2013-70B Rabbit Anti-GARP (Leucine-rich Repeat-containing Protein 32, Garpin, Glycoprotein A Repetitions Predominant, LRRC32, D11S833E)

Specifications
References
Clone Type
Polyclonal
Host
Rabbit
Source
Human
Isotype
IgG
Grade
Affinity Purified
Applications
FC WB
Crossreactivity
Hu Mk Mo Rt
Accession #
NP_001122394
Shipping Temp
Blue Ice
Storage Temp
-20°C

GARP, also known as leucine rich repeat containing 32, is a type I membrane protein with twenty-two leucine-rich repeats and a short intracytoplasmic region. GARP was identified based on the analysis of gene expression profiling of Treg and Th cells following TCR stimulation. Since GARP is expressed in CD4+, CD25+, FoxP3+ cells (Tregs), it may be a candidate for cell surface marker for Treg cells.

Applications
Suitable for use in Flow Cytometry and Western Blot. Other applications not tested.
Recommended Dilution
Western Blot: 0.5-1ug/ml Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Synthetic peptide corresponding to aa250-300 of human GARP.
Form
Supplied as a liquid in PBS, 0.05% BSA, 0.05% sodium azide.
Purity
Purified by Protein A affinity chromatography.
Specificity
Recognizes human GARP. Species Crossreactivity: monkey, mouse, rat. Species sequence homology: canine.
References
1. Ollendorff, V. et al. Cell Growth Differ. 5:213-219 (1994). 2. Wang, R. et al. PLoS ONE. 3(7):e2705 (2008). 3. Ollendorff, V. et al. Mammalian Genome. 2:195-200 (1992).
USBio References
No references available
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