Geminin inhibits DNA replication by preventing the incorporation of MCM complex into the prereplication complex (pre-RC). It is absent during the G1 phase, accumulates during the S, G2, and M phases, and disappears during the metaphase-anaphase transition. Geminin contains 212 amino acids and has a destruction box sequence (RRTLKVIQP). Its destruction at the metaphase-anaphase transition permits replication in the succeeding cell cycle.
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