Suitable for use in ELISA and Western Blot. Other applications not tested.

ELISA (Sandwich): 0.5-2ug/ml using 100ul/well antibody solution In conjunction with G2036-05A as a detection antibody, allows the detection of at least 0.2-0.4n/ml of recombinant human GDNF Western Blot: 0.1-0.2ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant human GDNF is 1.5-3ug/lane under non-reducing conditions. Optimal dilutions to be determined by the researcher.

Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Recombinant human GDNF, expressed in E. coli.

Supplied as a lyophilized powder in PBS, pH 7.2. Preservative free.|Reconstitute in 100ul sterile ddH2O.

Purified by immunoaffinity chromatography.

Recognizes human glial derived neurotrophic factor.