Four genes encoding glycine receptor alpha subunits have been identified, together with a single beta polypeptide. Each subunit consists of a large extracellular N-terminal region, four transmembrane domains, and a large cytoplasmic domain.

Suitable for use in Western Blot and Immunohistochemistry. Other applications not tested.

Immunohistochemistry (frozen): 1:1000 Western Blot: 1:1000, detects a band of ~48kD in rat spinal cord lysates. Optimal dilution determined by the researcher

Sheep anti rabbit IgG:HRP

Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Synthetic peptide corresponding to a region within the amino terminus of the alpha 1 subunit of rat glycine receptor.

Supplied as a lyophilized powder. No preservatives or stabilizers added. Reconstitute with 100ul sterile PBS. For long term storage the addition of 0.09% sodium azide is recommended.

Purified by affinity chromatography.

Recognizes the alpha 1 and alpha 2 subunits of rat glycine receptor. Species Crossreactivity: mouse and human