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G8168-01 Rabbit Anti-Glycine Transporter-1 (GLYT1) (Not for Export EU)

Specifications
References
Clone Type
Polyclonal
Host
Rabbit
Source
Rat
Isotype
IgG
Grade
Serum
Applications
E
Crossreactivity
Rt
Shipping Temp
Blue Ice
Storage Temp
-20°C

The glycine transporter belongs to a family of Na/Cl-dependent neurotransmitter transporters, predicted to contain as many as 12 transmembrane domains. Glycine receptor exists in 2 forms-GLYT1 and GLYT2. Form 1 has three other known isoforms (GLYT1a, GLYT1b, and GLYT1c) which may be produced by alternative spicing or promoter usage. Rodent Glyt1a and Glyt1b differ only by 10 amino acid at the N-terminus and expressed from the same gene. Although, the N-terminal part of mouse and rat GLYT1a is identical in mouse and rat, the N-terminus of GLYT1b are significantly different in these two species. The GLYT1c subtype has only been reported in humans. GLYT1a is expressed in CNS and peripheral organs. GLYT1b is localized in the CNS. Both neuron and Glial cells have GLYT1. More recent studies indicate that GLYt1b may not be brain specific. Immunolocalization studies on GLYT2 suggest that GLYT2 is responsible for terminating the neurotransmission at the strychnine synapses.

Applications
Suitable for use in ELISA. Western Blot, though not tested, may potentially be used as an application. Other applications not tested.
Recommended Dilution
Western Blot: 1:1000-1:5000 ELISA: 1:10,000-50,000 using 50-100ng G8168-03 Control peptide/well. Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Synthetic peptide corresponding to a 20aa sequence within the cytoplasmic, C-terminal domain of rat GLYT1 (KLH).
Form
Supplied as a liquid in 0.05% sodium azide.
Purity
Serum
Specificity
Recognizes rat GLYT1. Species sequence homology: 100%- human GLYT1a, GLYT1b, GLY1c and 95% with mouse and bovine GLYT1.
References
1. Kim KM et al (1994) Mol. Pharmacol. 45, 608; Smith KE et al (1992) Neuron 8, 927; Guatella J et al (1992) PNAS 89, 7189; Liu KR et al (1992) FEBS Lett. 305, 110; Jursky F et al (1996) J Neurochem. 67, 336
USBio References
No references available
Conjugates
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