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G8170-41P Rabbit Anti-Glycogen Synthase Kinase 3 B, phosphorylated (Ser9) (GSK3B, GSK-3 beta)

Specifications
References
Clone Type
Polyclonal
Host
Rabbit
Source
Rat
Swiss Prot
P18266
Isotype
IgG
Grade
Affinity Purified
Applications
WB
Crossreactivity
Mo Rt
Gene ID
84027
Shipping Temp
Blue Ice
Storage Temp
-20°C

Glycogen synthase kinase 3 (GSK3) is a serine/threonine kinase that is involved in the regulation of many signaling pathways. To date, 2 isoforms have been identified: GSK3α and GSK3β. Specifically, GSK3β has been shown to play a key inhibitory role in both the insulin and Wnt signaling pathways (Papkoff and Aikawa 1998). It has been suggested that Ser-9 phosphorylation underlies the inhibition of GSK3β by insulin (Sutherland et al., 1993).

Applications
Suitable for use in Western Blot. Other applications not tested.
Recommended Dilution
Western Blot: 1:1000, detects a band of ~46kD in rat cortex cell lysates. Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser9 of rat GSK3β, conjugated to KLH. Species Sequence Homology: bovine, canine, chicken, human, non-human pimate, Xenopus and zebrafish
Form
Supplied as a liquid in 10mM HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol.
Purity
Purified by immunoaffinity chromatography.
Specificity
Recognizes rat GSK3 beta when phosphorylated at Ser9. Weakly labels the ~51kD GSK3β band due to the high degree of homology between the 2 subunits. Species Crossreactivity: mouse
References
1. Sutherland C, Leighton IA, Cohen P (1993) Inactivation of glycogen synthase kinase 3 beta by phosphorylation: new kinase connections in insulin and growth factor signaling. Biochem J. Nov 15;296 (Pt. 1):15-9. PMID: 8250835.|2. Papkoff J, Aikawa M (1998)WNT-1 and HGF regulate GSK3b activity and beta catenin signaling in mammary epithelial cells. Biochem Biophys Res Commun. Jun 29;247(3):851-8. PMID: 9647782.
USBio References
No references available
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