GTP-binding proteins (G-proteins) play a critical role in signal transduction by coupling receptors to effectors. The intracellular consequences of G-protein activation include second messenger generation, protein phosphorylation, ion channel activation, gene induction, cell growth, and differentiation. Gi belongs to the pertussis toxin sensitive G-proteins. Gi consists of three different subtypes of alpha-subunits (alphai1, alphai2 and alphai3).

Suitable for use in Western Blot and Immunohistochemistry. Other applications not tested.

Immunohistochemistry: Paraffin sections require HIER with citrate buffer, pH 6.0 Optimal dilutions to be determined by the researcher.

Western Blot: L6 Cell Lysate Immunohistochemistry: Rat cerebellum

Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight™490 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Note: Applications are based on unconjugated antibody.

Synthetic peptide corresponding to aa345-354 of Gi alpha1 and aa346-355 of Gi alpha2.

Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight™490.

Purified

Recognizes human G protein subunit alphai1 and alpha2. Species Crossreactivity: mouse, rat and bovine