Granulocyte colony stimulating factor (G-CSF) is a 19.6kD haematopoietic growth factor and member of the CSF family of hormone-like glycoproteins, which stimulates the development of bone marrow progenitor cells and in particular those committed to neutrophil lineage. The ability of G-CSF to stimulate the proliferation/differentiation and function of neutrophil granulocyte progenitor cells and survival of mature neutrophils, has resulted in G-CSF becoming an important factor in patient treatment following allogeneic (bone marrow/stem cell) transplantation and chemotherapy.
Applications
Suitable for use in ELISA and Western Blot. Other applications have not been tested.
Recommended Dilutions
ELISA (Sandwich): 0.5-2ug/ml. (100ul/well) will detect at least 2000-4000pg/well of recombinant human G-CSF when used with G8950-26 as the capture antibody. Western Blot: 0.1-0.2ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hG-CSF is 1.5-3ng/lane, under either reducing or non-reducing conditions. Optimal dilutions to be determined by the researcher.
Storage and Stability
Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS, 0.1% BSA. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Immunogen
Recombinant human Granulocyte Colony Stimulating Factor (G-CSF), expressed in E. coli.
Form
Supplied as a lyophilized powder from PBS, pH 7.2. Labeled with Biotin. Reconstitute with sterile PBS containing 0.1% BSA to a concentration of 0.05mg/ml.
Purity
Purified by immunoaffinity chromatography.
Specificity
Recognizes human Granulocyte Colony Stimulating Factor (G-CSF).
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