Suitable for use in Western Blot, Immunoprecipitation, FLISA. Other applications not tested.
Recommended Dilution
Optimal dilutions to be determined by the researcher.
Storage and Stability
Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: APC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Note: Applications are based on unconjugated antibody.
Immunogen
GST fusion protein corresponding to residues 463-590 of bovine GRK 5.
Form
Supplied as a liquid in PBS, pH 7.2. Labeled with Allophycocyanin (APC).
Purity
Purified by ammonium sulfate precipitation
Specificity
Recognizes GRK 4, GRK 5 and GRK 6. Species Crossreactivity: Human, mouse and rat.
References
General Freedman, N.J., et al., J. Biol. Chem. 272: 17734-11743, 1997. Pitcher, J.A., et al., J. Biol. Chem. 271: 24907-24913, 1996. Nagayama, Y., et al., J. Biol. Chem. 271: 10143-10148, 1996. Application Oppermann, M., et al., Proc. Natl. Acad. Sci. USA 93: 7649-7654, 1996.