The Gb5 (GNB5 guanine nucleotide binding protein (G protein), beta 5) is a G protein subunit present predominantly in the central nervous system. This protein appears to be expressed in two forms, one found in the brain of 39kD and one found in the retina of 44kD. It has also been found that Gb5 associates tightly with RGS7, a member of the superfamily of negative regulators of G protein signaling. These complexes have been localized to most regions of the brain, whereas Gb1-4 exhibit no observable colocalization with RGS7, despite their widespread codistribution throughout similar neuronal networks. This antibody is specific for a peptide sequence within the D1 blade which is not found in Gb1-4.

Suitable for use in Western Blot. Other applications not tested.

Optimal dilutions to be determined by the researcher.

L6 Cell Lysate, plasma membranes from rat cerebrum.

Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight™650 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Note: Applications are based on unconjugated antibody.

Unique peptide sequence derived from the D1 blade of the Gb5 protein.

Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight™650.

Purified

Recognizes D1 area of human Gb5. Species Crossreactivity: mouse and rat.