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H1820-02-HRP Rabbit Anti-Haptoglobin (HP, BP, Haptoglobin alpha Chain, Haptoglobin beta Chain, HP2ALPHA2, HPA1S, MGC111141) (Azide free) (HRP)

Specifications
References
Clone Type
Polyclonal
Host
Rabbit
Source
Human
Swiss Prot
P00738
Conjugate
HRP
Isotype
IgG
Grade
Affinity Purified
Applications
E IHC WB
Crossreactivity
Hu Mo
Accession #
NP_005134.1
Shipping Temp
Blue Ice
Storage Temp
-20°C
Notes
Preservative Free

HP is a preproprotein, which is processed to yield both alpha and beta chains, which subsequently combine as a tetramer to produce haptoglobin. Haptoglobin functions to bind free plasma hemoglobin, which allows degradative enzymes to gain access to the hemoglobin, while at the same time preventing loss of iron through the kidneys and protecting the kidneys from damage by hemoglobin. Mutations in this gene and/or its regulatory regions cause ahaptoglobinemia or hypohaptoglobinemia.

Applications
Suitable for use in ELISA and Western Blot and Immunohistochemistry. Other applications not tested.
Recommended Dilution
Immunohistochemistry: Formalin-fixed, paraffin-embedded sections Optimal dilutions to be determined by the researcher.
Storage and Stability
Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Note: Applications are based on unconjugated antibody.
Immunogen
KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 295-322 in the central region of human Haptoglobin, Swiss/UniProt Accession: P00738.
Form
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with horseradish peroxidase (HRP).
Purity
Purified by Protein A and peptide affinity chromatography.
Specificity
Recognizes human Haptoglobin. Species Crossreactivity: mouse
References
1. Ryndel,M., et.al., Clin. Chim. Acta 411(7-8), 500-504 (2010). 2. Igl,W., PLoS Genet. 6(1), E1000798 (2010).
USBio References
No references available
Conjugates
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