MaxLight™550 is a new Yellow-Green photostable dye conjugate comparable to Alexa Fluor™546, 555, DyLight™549 , Cy3™, TRITC and offers better labeling efficiency, brighter imaging and increased immunodetection. Absorbance (550nm); Emission (575nm); Extinction Coefficient 150,000.
Applications
Suitable for use in FLISA. Not suitable for use in Western blot. Other applications not tested.
Recommended Dilution
FLISA: 1:500 (employing native viral lysates). Optimal dilutions to be determined by the researcher.
Positive Control
H1920-04: Hepatitis C Virus Core, 22kD, aa2-192, Recombinant (HCV)
Storage and Stability
Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight™550 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Note: Applications are based on unconjugated antibody.
Immunogen
Recombinant Hepatitis C Virus Core Antigen (HCV)
Form
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight™550.
Purity
Purified by affinity chromatography.
Specificity
Recognizes Hepatitis C Virus Core antigen. Epitope: aa1-80 of HCV core antigen.