Suitable for use in ELISA and Western Blot. Other applications not tested.

ELISA: 1:500-1:1000; Tested using histidine tagged ubiquitin. Western Blot: 1:1000; Tested using His-tagged recombinant protein control. H5099-16A has been shown not to crossreact with any endogenous products when using bacterial extracts. Some crossreactivity may be seen with extracts of insect or mammalian cells. Optimal dilutions to be determined by the researcher.

S7973-97B: Streptavidin (HRP)

NS1 myeloma cells with spleen cells from Balb/c mice.

Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

PAX6 transcription factor linked to histidine tag.

Supplied as a liquid in PBS, pH 7.4, 0.09% sodium azide. Labeled with Biotin.

Purified by Protein G affinity chromatography from tissue culture supernatant.

Recognizes proteins containing the motif H-H-H-H-H-H and proteins containing histidine tags. Reacts against all His tagged proteins so far tested. May be used to track the purification of proteins expressed in bacteria, yeast or insect cells.