Suitable for use in Western Blot, Immunohistochemistry and Immunocytochemistry. Not suitable for ELISA. Other applications not tested.

Immunohistochemistry: 1:1000 detects mitotic cells in C. elegans embryo sections. Immunocytochemistry: Positive staining of mitotic cells in cultures of HeLa cells undergoing exponential growth. Western Blot: 0.005-0.03ug/ml. Detects phosphorylated Histone H3 in acid extracted proteins from colcemid-arrested HeLa cells. Acid-extracted proteins from exponentially growing HeLa cells were resolved by electrophoresis, transferred to nitrocellulose and probed with H5110-14M at 0.03ug/ml. Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and a chemiluminescence detection system. Optimal dilutions to be determined by the researcher.

I1904-06C: IgG, H&L (HRP) (X-Adsorbed) Pab Gt xMo I1904-06H IgG, H&L (HRP) (X-Adsorbed) Pab Gt xMo

UV-treated 293 cell extracts, UV-treated HeLa cell extracts, breast cancer tissue, HEPG2 cell extracts.

May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Synthetic peptide corresponding to aa7-20, ARK[pS]TGGKAPRKQL-C of human histone H3 with a C-terminal cyteine added for conjugation purposes.

Supplied as a liquid in 0.1M Tris-glycine, 0.15M sodium chloride, pH 7.4, 0.05% sodium azide.

Purified by Protein G affinity chromatography.

Recognizes human Histone H3 phosphorylated at Serine 10 (Mr 17kD). Species Crossreactivity: C. elegans. Broad species crossreactivity expected due to sequence conservation.