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H6098-39F2 Mouse Anti-HLA Class 1 Antigen ABC (Biotin)

Specifications
References
Clone Type
Monoclonal
Host
Mouse
Source
Human
Conjugate
Biotin
Isotype
IgG2a
Clone Number
3H2211 (W6/32)
Grade
Affinity Purified
Applications
FC
Crossreactivity
Bo Fe Hu Mc Mk
Shipping Temp
Blue Ice
Storage Temp
-20°C
HLA Class 1 A, HLA A, HLA Class 1 B, HLA B, HLA Class 1 C, HLA C, MHC Class I HLA A, MHC Class I HLA B, MHC Class I HLA C

The major histocompatibility complex (MHC) is a cluster of genes that are important in the immune response to infections. In humans, this complex is referred to as the human leukocyte antigen (HLA) region. There are 3 major MHC class I proteins encoded by the HLA which are HLA A, HLA B and HLA C. These proteins are found on the surface of almost all nucleated somatic cells.

HLA-ABC antigen is present on most nucleated cells of humans and old world monkeys. HLA Class 1 Antigen ABC is a monomorphic determinant of human Class I HLA molecules (HLA-A,B,C) associated with b2-microblogulin. It is expressed at the surface of human lymphoblastic B cells.
Human Leukocyte Antigen (HLA) class I which is also known as human class I Major Histocompatibility Complex (MHC). HLA class I is encoded by three HLA alleles: HLA-A, HLA-B and HLA-C. HLA class I is a protein dimer composed of an alpha chain (Mr =44kD) and a beta chain which is also known as b2 microglobulin (Mr =12kD). The smaller beta chain bears homology with immunoglobulin molecules. The alpha chain contains four distinct regions: a cytoplasmic region located at the carboxyl terminus of the protein, followed by a transmembrane region, an immunoglobulin-like region and a peptide-binding region located at the amino terminus. This peptide binding region contains a cleft which can bind peptides consisting of 9-11 amino acid residues. HLA class 1 specifically targets the bound peptide to T cells while interacting with the T-cell receptor. HLA class 1 appears to preferentially target CD8+ T cells, whereas HLA class II preferentially targets CD4+ T cells.
Applications
Suitable for use in Flow Cytometry. Other applications have not been tested.
Recommended Dilution
Flow Cytometry: Neat-1:5. Use 20ul to label 10e6 cells or 100ul whole blood. Optimal working dilutions to be determined by researcher.
Recommended Secondary Reagents
S7973-78: Streptavidin (PE) S7973-92F2: Streptavidin (PE) S7973-96A: Streptavidin (FITC) S7973-96B: Streptavidin (FITC)
Recommended Negative Controls
I1904-83V1: IgG2a Negative Control (Biotin)
Hybridoma
Spleen cells from immunized BALB/c mice fused with mouse NS1/1-Ag4.1 myeloma cells
Storage and Stability
Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Purified human tonsil lymphocyte membranes.
Form
Supplied as a liquid in PBS, pH 7.4, 1% BSA, 0.09% sodium azide. Labeled with Biotin.
Purity
Purified by Protein G affinity chromatography from tissue culture supernatant.
Specificity
Recognizes an antigenic determinant shared among products of the human HLA A, B and C loci. Recognizes a conformational epitope, reacting with HLA class I alpha3 and alpha2 domains (6). Species crossreactivity: Macaque, bovine, Cynomolgus monkey, Baboon, Rhesus Monkey, Chimpanzee, Gorilla, Shrew. Does not react with goat, canine, guinea pig, rabbit, mouse, chicken or amphibia.
References
1. Barnstable, C. J., et al. (1978) Production of monoclonal antibodies to group A erythrocytes, HLA and other human cell surface antigens - new tools for genetic analysis. Cell. 14: 9 - 20. 2. Burrone, O. R. et al. (1982) Control of HLA-A, B, C sythesis and expression in interferon cells. EMBO J. 1; 345 - 349. 3. Jacobsen, C. N. et al. (1993) Reactivities of 20 anti-human monclonal antibodies with leucocytes from ten different animal species. Vet. Immunopathol. 39: 461 - 466. 4. Brodsky, F.M. et al. (1982). Evolution of HLA antigenic determinants: species cross reactions of monoclonal antibodies. Immunogenetics 15: 151-166. 5. Neefjes, J.J. et al. (1986). A biochemical characterization of feline MHC products: unusually high expression of class II antigens on peripheral blood lymphocytes. Immunogenetics 23: 341-347. 6. Yoshino, N. et al. (2000) Upgrading of flow cytometric analysis for absolute counts, cytokines and other antigenic molecules of Cynomolgus monkeys (Macaca fascicularis) by using anti-human cross-reactive antibodies. Exp. Anim. 49 (2): 97-110. 7. Stern, P. et al. (1987). Class I-like MHC molecules expressed by baboon placental synctiotrophoblast. Journal of Immunology. 138 (4): 1088 - 1091.
USBio References
No references available
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