HLA-ABC antigen is present on most nucleated cells of humans and old world monkeys. HLA Class 1 Antigen ABC is a monomorphic determinant of human Class I HLA molecules (HLA-A,B,C) associated with b2-microblogulin. It is expressed at the surface of human lymphoblastic B cells.

Human Leukocyte Antigen (HLA) class I which is also known as human class I Major Histocompatibility Complex (MHC). HLA class I is encoded by three HLA alleles: HLA-A, HLA-B and HLA-C. HLA class I is a protein dimer composed of an alpha chain (Mr =44kD) and a beta chain which is also known as b2 microglobulin (Mr =12kD). The smaller beta chain bears homology with immunoglobulin molecules. The alpha chain contains four distinct regions: a cytoplasmic region located at the carboxyl terminus of the protein, followed by a transmembrane region, an immunoglobulin-like region and a peptide-binding region located at the amino terminus. This peptide binding region contains a cleft which can bind peptides consisting of 9-11 amino acid residues. HLA class 1 specifically targets the bound peptide to T cells while interacting with the T-cell receptor. HLA class 1 appears to preferentially target CD8+ T cells, whereas HLA class II preferentially targets CD4+ T cells.

Suitable for use in Immunohistochemistry. Other applications not tested.

Immunohistochemistry: frozen sections with acetone fixation. The epitope of this antibody is reported to be sensitive to formaldehyde and tissue processing. Optimal dilutions to be determined by the researcher.

May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. Labeled with horseradish peroxidase (HRP). Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. HRP conjugates are sensitive to light.

Purified human tonsil lymphocyte membranes.

Supplied as a liquid in PBS, pH 7.4, 0.01% thiomerosal, 50% HRP stabilizer. Labeled with horseradish peroxidase (HRP).

Purified by Protein G affinity chromatography.

Recognizes an antigenic determinant shared among products of the human HLA A, B and C loci. Recognizes a conformational epitope, reacting with HLA class I alpha3 and alpha2 domains (6). Species crossreactivity: Macaque, bovine, Cynomolgus monkey, Baboon, Rhesus Monkey, Chimpanzee, Gorilla, Shrew. Does not react with goat, canine, guinea pig, rabbit, mouse, chicken or amphibia.