The major histocompatibility complex (MHC) is a cluster of genes that are important in the immune response to infections. In humans, this complex is referred to as the human leukocyte antigen (HLA) region. There are 3 major MHC class II proteins encoded by the HLA which are HLA DP, HLA DQ and HLA DR.

Suitable for use in Flow Cytometry and Immunohistochemistry. Other applications have not been tested.

Immunohistochemistry: Frozen and paraffin sections (PLP periodate-lysine- paraformaldehyde fixation) Optimal dilutions to be determined by researcher.

Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight™550 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Note: Applications are based on unconjugated antibody.

EHR-B Ramos cells

Supplied as a liquid in PBS, pH 7.2. Labeled with MaxLight™550.

Purified by Protein G affinity chromatography.

Recognizes a monomorphic determinant of human HLA-DR, expressed by B lymphocytes, monocytes and activated T cells. Reported to be cross reactive with mouse H-2 haplotypes b, d and q expressed by mouse strains including C57BL/6, DBA, and NZB (1).