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H7980-36 Anti-Hyaluronic Acid Binding Protein 1, Recombinant, Mouse (Hyaluronan Binding Protein 1, HABP1, C1qBP, C1q-R, Human complement component 1, q subcomponent binding protein)

Specifications
References
Grade
Highly Purified
Accession #
NP_031599
Shipping Temp
Blue Ice
Storage Temp
-20°C

Hyaluronan binding protein 1 (HABP1), also known as C1qBP/C1qR and p32, is a ubiquitous acidic glycoprotein that functions in spermatogenesis and as a receptor for proinflammatory molecules. HABP1 is synthesized with a 71 amino acid (aa) N-terminal preproprotein and a 208aa mature region. The 3kD mature mouse HABP1, which contains a MAM33-like sequence, shares 90% and 99% aa sequence identity with human and rat HABP1, respectively. HABP1 assembles into a doughnut shaped trimer, with negatively charged residues asymmetrically distributed on one face lining the channel of the complex. HABP1 can be cleaved by cell surface MMP-14/MT1-MMP, a protease important in angiogenesis and tumor metastasis. Cell surface HABP1 binds a wide range of extracellular molecules, including hyaluronan, vitronectin, complement component C1q, HMW kininogen, and bacterial and viral proteins. Within the cell, HABP1 binds to molecules containing the C1q globular domain, multiple isoforms of PKC, mitochondrial Hrk, the cytoplasmic tails of adrenergic and GABA-A receptors, the mRNA splicing factor ASF/SF2, and the CBF transcription factor. Apoptosis and direct phosphorylation by Erk1/2 induces HABP1 translocation to the nucleus.

A DNA sequence encoding the mature mouse HABP1 (Leu 72-Gln 279; Accession # NP_031599), fused with 2X Met at the N-terminus and a six-histidine tag at the C-terminus, was expressed in E. coli.
Predicted Molecular Mass
~24.8kD
Activity
Measured by its ability to bind human C1q.
Storage and Stability
Lyophilized powder may be stored at -20°C. Stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Source
Recombinant, E. coli
Concentration
~0.1mg/ml (after reconstitution)
Form
Supplied as a lyophilized powder in PBS. Reconstitute with 500ul sterile PBS.
Important Note
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Form
Supplied as a lyophilized powder in PBS. Reconstitute with 500ul sterile PBS.
Purity
≥95%, as determined by SDS-PAGE under reducing conditions and visualized by silver stain. Endotoxin: ≤1EU/1ug
References
1. Thakur, S.C. et al., 2006, J. Androl. 27:604. 2. Peerschke, E.I.B. and B. Ghebrehiwet, 2007, Immunobiology 212:333. 3. Lynch, N.J. et al., 1997, FEBS Lett. 418:111. 4. Jiang, J. et al., 1999, Proc. Natl. Acad. Sci. 96:3572. 5. Rozanov, D.V. et al., 2002, J. Biol. Chem. 277:9318. 6. Deb, T.B. and K. Datta, 1996, J. Biol. Chem. 271:2206. 7. Lim, B.L. et al., 1996, J. Biol. Chem. 271:26739. 8. Grebrehiwet, B. et al., 1994, J. Exp. Med. 179:1809. 9. Waggoner, S.N. et al., 2007, J. Leukoc. Biol. 82 epub. 10. Innamorati, G. et al., 2006, Cell. Signal. 18:761. 11. Robles-Flores, M. et al., 2002, J. Biol. Chem. 277:5247. 12. Sunayama, J. et al. (1994) Cell Death Differ. 11:771. 13. Pupo, A.S. and K.P. Minneman (2003) J. Recept. Signal Transduct. Res. 23:185. 14. Schaerer, M.T. et al. (2001) J. Biol. Chem. 276:26597. 15. Peterson­Mahrt, S.K. et al. (1999) EMBO J. 18:1014. 16. Chattopadhyay, C. et al. (2004) Nucleic Acids Res. 32:3632. 17. Majumdar, M. et al. (2002) Biochem. Biophys. Res. Commun. 291:829.
USBio References
No references available
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