NO is synthesized by L-arginine, oxygen, and NADPH by three known isoforms of heme-containing flavoproteins termed NO synthase (NOS, I-III, MW ~130-160kD). One group of enzyme is constitutive, agonist-triggered, and dependent on Ca2+/Calmodulin and is inhibited by L-arginine analogues (L-NNA, L-NMMA). It is found in endothelium, adrenal glands, brain and platelets. The other principle group is inducible, Ca2+/Calmodulin-independent, and inhibited by NMMA and L-NNA. It has been found in macrophage, hepatocytes, tumor cells, vascular smooth muscle and endothelial cells. Analyses of cDNA clones have identified three distinct NOS genes in mammals: neuronal (nNOS/bNOS/NOS-I), endothelial (eNOS/NOS-III), and macrophage (mNOS/iNOS/NOS-II). Both nNOS and eNOS are constitutive and the mNOS/iNOS is inducible. Sequence homology among different cloned isoforms is ~ 50%.

Suitable for use in ELISA, Western Blot. Other applications not tested.

ELISA: Control peptide should be coated at 1ug/ml. Western Blot: 1:100-1:1000. Recognizes 130kD protein.

Optimal dilutions to be determined by the researcher.

Control Peptide: I0800-09A

May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Synthetic peptide mapping to 14aa within the C-terminal of mouse nitric oxide synthase (KLH coupled). Human, rat and mouse iNOS/NOS-2 are ~1145aa proteins.

Supplied as a liquid, serum, 0.09% sodium azide, 40% glycerol.

Serum

Recognizes mouse inducible nitric oxide synthase (iNOS, NOS-II). No significant sequence homology of iNOS-P is seen with NOS-1/NOS-3 or other proteins. Species homology: Human and rat (85%, 12/14 aa).