Suitable for use in ELISA for detection of total IgE level and IgE antibodies. Other applications have not been tested.
Recommended Dilution
Indirect ELISA: 1:500,000 Optimal dilutions to be determined by the researcher.
Matched Pair
Capture: I1900-06A Detection: I1900-06
Hybridoma
Sp2/0 myeloma cells with spleen cells of Balb/c mice
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
IgE from myelomatous human serum
Form
Supplied as a liquid in PBS, pH 7.4, 0.09% sodium azide.
Purity
Purified by Protein A affinity chromatography.
Specificity
Recognizes human IgE. Specific for C-epsilon-2-domain.
References
1. Allergol. et Immunopathol. 1978, vol 1, pp. 423-426 2. Allergy and Clin. Immunol. News, 1992, vol.4, 21-25
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