Suitable for use in ELISA and Western Blot. Other applications not tested.

ELISA: 0.5ug/ml Optimal dilutions to be determined by the researcher.

Spleen cells from immunized BALB/c mice were fused with cells of the mouse SP2/0 myeloma cell line

4x10e9M-1

Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Rat IgE myeloma protein (IR162, IR1016, IR2 ,IR410)

Supplied as a liquid in PBS, 0.01% thimerosal, 50% glycerol. Labeled with Horseradish Peroxidase (HRP).

Purified by affinity chromatography from tissue culture supernatent

Recognizes rat epsilon heavy chain of immunoglobulin IgE and does not cross-react with other classes of rat immunoglobulin.