Suitable for use in Immunomicroscopy and FLISA. Other applications have not been tested.

Optimal dilutions to be determined by the researcher.

Fluorescein isothiocyanate (FITC) (Molecular Weight 390 daltons)

495 nm

528 nm

3.6 moles FITC per mole of Goat IgG

Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Bovine IgG F(c) fragment

Supplied as a liquid in PBS, pH 7.6, 15mg/ml BSA, 0.05% sodium azide, 0.01% thimerosal. Labeled with Fluorescein isothiocyanate (FITC).

Purified by immunoaffinity chromatography.

Assay by immunoelectrophoresis resulted in a single precipitin arc against anti fluorescein, anti goat serum, bovine igG, bovine IgGFc and bovine serum. No reaction was observed against bovine IgGF(ab’)2.