Applications
Suitable for use in ELISA, Immunoprecipitation, Immunodiffusion and conjugation. Other applications not tested.
Recommended Dilution
ELISA: 1:50,000 Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Immunogen
Guinea Pig IgG whole molecule
Form
Supplied as a liquid in PBS, pH 7.2, 0.01% sodium azide. No stabilizing proteins added.
Purity
Purified by immunoaffinity chromatography.
Specificity
Recognizes guinea pig IgG (H&L), X-adsorbed. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, guinea pig IgG and guinea pig Serum. Does not crossreact with Bovine, Chicken, Goat, Hamster, Human, Mouse, Rabbit, Rat and Sheep Serum Proteins.